Long-term viability of isolated bovine adrenal medullary chromaffin cells following intrastriatal transplantation
Identifieur interne : 000371 ( Main/Corpus ); précédent : 000370; suivant : 000372Long-term viability of isolated bovine adrenal medullary chromaffin cells following intrastriatal transplantation
Auteurs : Sherry B. Schueler ; Jacqueline Sagen ; George D. Pappas ; Jeffrey H. KordowerSource :
- Cell Transplantation [ 0963-6897 ] ; 1994.
Abstract
Adrenal medullary grafts generally exhibit poor viability when grafted into the striatum. Previous work in our laboratory demonstrated that chromaffin cells can survive well for up to 2 mo following grafting into the intact rat striatum after cells are isolated from the nonchromaffin supporting cells (fibroblasts and endothelial cells) of the adrenal medulla. The aim of the present study was to assess the long-term viability of isolated bovine chromaffin cells following grafting into the intact rat striatum. The viability of grafted bovine adrenal medullary chromaffin cells was compared in rats receiving either (a) perfused adrenal medulla; (b) isolated chromaffin cells; or (c) isolated chromaffin cells that were subsequently recombined with their nonchromaffin supporting cells. One year postimplantation, all graft types which included fibroblasts and endothelial cells were infiltrated with macrophages and demonstrated an abundance of cellular debris. No viable chromaffin cells were observed. In contrast, healthy tyrosine hydroxylase (TH) and dopamine beta hydroxylase (DβH) immunoreactive chromaffin cells survived for 1 yr posttransplantation when grafted in isolation from the nonchromaffin constituents of the adrenal medulla. Good xenograft survival was achieved in this group despite the fact that these rats were only immunosuppressed for 1 mo postimplantation. Grafted cells demonstrated morphological characteristics of chromaffin cells in situ and these implants were not accompanied by macrophage infiltration. These data demonstrate that long-term survival of chromaffin cells can be achieved following intra-striatal implantation and the viability of grafted chromaffin cells is dependent upon the removal of the nonchromaffin supporting cells.
Url:
DOI: 10.1016/0963-6897(94)00939-H
Links to Exploration step
ISTEX:49F400E7DD52410E715273CE694FB97B879231EELe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Long-term viability of isolated bovine adrenal medullary chromaffin cells following intrastriatal transplantation</title><author><name sortKey="Schueler, Sherry B" sort="Schueler, Sherry B" uniqKey="Schueler S" first="Sherry B." last="Schueler">Sherry B. Schueler</name><affiliation><mods:affiliation>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</mods:affiliation></affiliation></author><author><name sortKey="Sagen, Jacqueline" sort="Sagen, Jacqueline" uniqKey="Sagen J" first="Jacqueline" last="Sagen">Jacqueline Sagen</name><affiliation><mods:affiliation>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</mods:affiliation></affiliation></author><author><name sortKey="Pappas, George D" sort="Pappas, George D" uniqKey="Pappas G" first="George D." last="Pappas">George D. Pappas</name><affiliation><mods:affiliation>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</mods:affiliation></affiliation></author><author><name sortKey="Kordower, Jeffrey H" sort="Kordower, Jeffrey H" uniqKey="Kordower J" first="Jeffrey H." last="Kordower">Jeffrey H. Kordower</name><affiliation><mods:affiliation>To whom correspondence should be addressed.</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Neurological Sciences, Rush Presbyterian Medical Center, 2242 West Harrision Street, Chicago, IL 60612 USA</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:49F400E7DD52410E715273CE694FB97B879231EE</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1016/0963-6897(94)00939-H</idno><idno type="url">https://api.istex.fr/document/49F400E7DD52410E715273CE694FB97B879231EE/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">000371</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Long-term viability of isolated bovine adrenal medullary chromaffin cells following intrastriatal transplantation</title><author><name sortKey="Schueler, Sherry B" sort="Schueler, Sherry B" uniqKey="Schueler S" first="Sherry B." last="Schueler">Sherry B. Schueler</name><affiliation><mods:affiliation>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</mods:affiliation></affiliation></author><author><name sortKey="Sagen, Jacqueline" sort="Sagen, Jacqueline" uniqKey="Sagen J" first="Jacqueline" last="Sagen">Jacqueline Sagen</name><affiliation><mods:affiliation>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</mods:affiliation></affiliation></author><author><name sortKey="Pappas, George D" sort="Pappas, George D" uniqKey="Pappas G" first="George D." last="Pappas">George D. Pappas</name><affiliation><mods:affiliation>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</mods:affiliation></affiliation></author><author><name sortKey="Kordower, Jeffrey H" sort="Kordower, Jeffrey H" uniqKey="Kordower J" first="Jeffrey H." last="Kordower">Jeffrey H. Kordower</name><affiliation><mods:affiliation>To whom correspondence should be addressed.</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Neurological Sciences, Rush Presbyterian Medical Center, 2242 West Harrision Street, Chicago, IL 60612 USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell Transplantation</title><title level="j" type="abbrev">CTR</title><idno type="ISSN">0963-6897</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">4</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="55">55</biblScope><biblScope unit="page" to="64">64</biblScope></imprint><idno type="ISSN">0963-6897</idno></series><idno type="istex">49F400E7DD52410E715273CE694FB97B879231EE</idno><idno type="DOI">10.1016/0963-6897(94)00939-H</idno><idno type="PII">0963-6897(94)00939-H</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0963-6897</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Adrenal medullary grafts generally exhibit poor viability when grafted into the striatum. Previous work in our laboratory demonstrated that chromaffin cells can survive well for up to 2 mo following grafting into the intact rat striatum after cells are isolated from the nonchromaffin supporting cells (fibroblasts and endothelial cells) of the adrenal medulla. The aim of the present study was to assess the long-term viability of isolated bovine chromaffin cells following grafting into the intact rat striatum. The viability of grafted bovine adrenal medullary chromaffin cells was compared in rats receiving either (a) perfused adrenal medulla; (b) isolated chromaffin cells; or (c) isolated chromaffin cells that were subsequently recombined with their nonchromaffin supporting cells. One year postimplantation, all graft types which included fibroblasts and endothelial cells were infiltrated with macrophages and demonstrated an abundance of cellular debris. No viable chromaffin cells were observed. In contrast, healthy tyrosine hydroxylase (TH) and dopamine beta hydroxylase (DβH) immunoreactive chromaffin cells survived for 1 yr posttransplantation when grafted in isolation from the nonchromaffin constituents of the adrenal medulla. Good xenograft survival was achieved in this group despite the fact that these rats were only immunosuppressed for 1 mo postimplantation. Grafted cells demonstrated morphological characteristics of chromaffin cells in situ and these implants were not accompanied by macrophage infiltration. These data demonstrate that long-term survival of chromaffin cells can be achieved following intra-striatal implantation and the viability of grafted chromaffin cells is dependent upon the removal of the nonchromaffin supporting cells.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Sherry B. Schueler</name><affiliations><json:string>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</json:string></affiliations></json:item><json:item><name>Jacqueline Sagen</name><affiliations><json:string>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</json:string></affiliations></json:item><json:item><name>George D. Pappas</name><affiliations><json:string>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</json:string></affiliations></json:item><json:item><name>Jeffrey H. Kordower</name><affiliations><json:string>To whom correspondence should be addressed.</json:string><json:string>Department of Neurological Sciences, Rush Presbyterian Medical Center, 2242 West Harrision Street, Chicago, IL 60612 USA</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Xenograft</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Chromaffin cell</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Intrastriatal transplantation</value></json:item></subject><language><json:string>eng</json:string></language><abstract>Adrenal medullary grafts generally exhibit poor viability when grafted into the striatum. Previous work in our laboratory demonstrated that chromaffin cells can survive well for up to 2 mo following grafting into the intact rat striatum after cells are isolated from the nonchromaffin supporting cells (fibroblasts and endothelial cells) of the adrenal medulla. The aim of the present study was to assess the long-term viability of isolated bovine chromaffin cells following grafting into the intact rat striatum. The viability of grafted bovine adrenal medullary chromaffin cells was compared in rats receiving either (a) perfused adrenal medulla; (b) isolated chromaffin cells; or (c) isolated chromaffin cells that were subsequently recombined with their nonchromaffin supporting cells. One year postimplantation, all graft types which included fibroblasts and endothelial cells were infiltrated with macrophages and demonstrated an abundance of cellular debris. No viable chromaffin cells were observed. In contrast, healthy tyrosine hydroxylase (TH) and dopamine beta hydroxylase (DβH) immunoreactive chromaffin cells survived for 1 yr posttransplantation when grafted in isolation from the nonchromaffin constituents of the adrenal medulla. Good xenograft survival was achieved in this group despite the fact that these rats were only immunosuppressed for 1 mo postimplantation. Grafted cells demonstrated morphological characteristics of chromaffin cells in situ and these implants were not accompanied by macrophage infiltration. These data demonstrate that long-term survival of chromaffin cells can be achieved following intra-striatal implantation and the viability of grafted chromaffin cells is dependent upon the removal of the nonchromaffin supporting cells.</abstract><qualityIndicators><score>7.952</score><pdfVersion>1.2</pdfVersion><pdfPageSize>612 x 828 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>3</keywordCount><abstractCharCount>1773</abstractCharCount><pdfWordCount>5775</pdfWordCount><pdfCharCount>39261</pdfCharCount><pdfPageCount>10</pdfPageCount><abstractWordCount>246</abstractWordCount></qualityIndicators><title>Long-term viability of isolated bovine adrenal medullary chromaffin cells following intrastriatal transplantation</title><pii><json:string>0963-6897(94)00939-H</json:string></pii><genre><json:string>research-article</json:string></genre><serie><volume>65</volume><pages><last>328</last><first>305</first></pages><genre></genre><language><json:string>unknown</json:string></language><title>Mayo Clin. Proc.</title></serie><host><volume>4</volume><pii><json:string>S0963-6897(00)X0012-7</json:string></pii><pages><last>64</last><first>55</first></pages><issn><json:string>0963-6897</json:string></issn><issue>1</issue><genre><json:string>Journal</json:string></genre><language><json:string>unknown</json:string></language><title>Cell Transplantation</title><publicationDate>1995</publicationDate></host><categories><wos><json:string>MEDICINE, RESEARCH & EXPERIMENTAL</json:string><json:string>TRANSPLANTATION</json:string><json:string>CELL & TISSUE ENGINEERING</json:string><json:string>CELL BIOLOGY</json:string></wos></categories><publicationDate>1994</publicationDate><copyrightDate>1995</copyrightDate><doi><json:string>10.1016/0963-6897(94)00939-H</json:string></doi><id>49F400E7DD52410E715273CE694FB97B879231EE</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/49F400E7DD52410E715273CE694FB97B879231EE/fulltext/pdf</uri></json:item><json:item><original>true</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/49F400E7DD52410E715273CE694FB97B879231EE/fulltext/txt</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/49F400E7DD52410E715273CE694FB97B879231EE/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/49F400E7DD52410E715273CE694FB97B879231EE/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Long-term viability of isolated bovine adrenal medullary chromaffin cells following intrastriatal transplantation</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1995</date></publicationStmt><notesStmt><note type="content">Section title: Original contribution</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Long-term viability of isolated bovine adrenal medullary chromaffin cells following intrastriatal transplantation</title><author><persName><forename type="first">Sherry B.</forename><surname>Schueler</surname></persName><affiliation>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</affiliation></author><author><persName><forename type="first">Jacqueline</forename><surname>Sagen</surname></persName><affiliation>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</affiliation></author><author><persName><forename type="first">George D.</forename><surname>Pappas</surname></persName><affiliation>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</affiliation></author><author><persName><forename type="first">Jeffrey H.</forename><surname>Kordower</surname></persName><affiliation>To whom correspondence should be addressed.</affiliation><affiliation>Department of Neurological Sciences, Rush Presbyterian Medical Center, 2242 West Harrision Street, Chicago, IL 60612 USA</affiliation></author></analytic><monogr><title level="j">Cell Transplantation</title><title level="j" type="abbrev">CTR</title><idno type="pISSN">0963-6897</idno><idno type="PII">S0963-6897(00)X0012-7</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994"></date><biblScope unit="volume">4</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="55">55</biblScope><biblScope unit="page" to="64">64</biblScope></imprint></monogr><idno type="istex">49F400E7DD52410E715273CE694FB97B879231EE</idno><idno type="DOI">10.1016/0963-6897(94)00939-H</idno><idno type="PII">0963-6897(94)00939-H</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1995</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Adrenal medullary grafts generally exhibit poor viability when grafted into the striatum. Previous work in our laboratory demonstrated that chromaffin cells can survive well for up to 2 mo following grafting into the intact rat striatum after cells are isolated from the nonchromaffin supporting cells (fibroblasts and endothelial cells) of the adrenal medulla. The aim of the present study was to assess the long-term viability of isolated bovine chromaffin cells following grafting into the intact rat striatum. The viability of grafted bovine adrenal medullary chromaffin cells was compared in rats receiving either (a) perfused adrenal medulla; (b) isolated chromaffin cells; or (c) isolated chromaffin cells that were subsequently recombined with their nonchromaffin supporting cells. One year postimplantation, all graft types which included fibroblasts and endothelial cells were infiltrated with macrophages and demonstrated an abundance of cellular debris. No viable chromaffin cells were observed. In contrast, healthy tyrosine hydroxylase (TH) and dopamine beta hydroxylase (DβH) immunoreactive chromaffin cells survived for 1 yr posttransplantation when grafted in isolation from the nonchromaffin constituents of the adrenal medulla. Good xenograft survival was achieved in this group despite the fact that these rats were only immunosuppressed for 1 mo postimplantation. Grafted cells demonstrated morphological characteristics of chromaffin cells in situ and these implants were not accompanied by macrophage infiltration. These data demonstrate that long-term survival of chromaffin cells can be achieved following intra-striatal implantation and the viability of grafted chromaffin cells is dependent upon the removal of the nonchromaffin supporting cells.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Xenograft</term></item><item><term>Chromaffin cell</term></item><item><term>Intrastriatal transplantation</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1994-09-06">Registration</change><change when="1994">Published</change></revisionDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>CTR</jid><aid>9400939H</aid><ce:pii>0963-6897(94)00939-H</ce:pii><ce:doi>10.1016/0963-6897(94)00939-H</ce:doi><ce:copyright type="unknown" year="1995"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Original contribution</ce:textfn></ce:dochead><ce:title>Long-term viability of isolated bovine adrenal medullary chromaffin cells following intrastriatal transplantation</ce:title><ce:author-group><ce:author><ce:given-name>Sherry B.</ce:given-name><ce:surname>Schueler</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Jacqueline</ce:given-name><ce:surname>Sagen</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>George D.</ce:given-name><ce:surname>Pappas</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Jeffrey H.</ce:given-name><ce:surname>Kordower</ce:surname><ce:cross-ref refid="COR1"><ce:sup>1</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Department of Neurological Sciences, Rush Presbyterian Medical Center, 2242 West Harrision Street, Chicago, IL 60612 USA</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>1</ce:label><ce:text>To whom correspondence should be addressed.</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="6" month="9" year="1994"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Adrenal medullary grafts generally exhibit poor viability when grafted into the striatum. Previous work in our laboratory demonstrated that chromaffin cells can survive well for up to 2 mo following grafting into the intact rat striatum after cells are isolated from the nonchromaffin supporting cells (fibroblasts and endothelial cells) of the adrenal medulla. The aim of the present study was to assess the long-term viability of isolated bovine chromaffin cells following grafting into the intact rat striatum. The viability of grafted bovine adrenal medullary chromaffin cells was compared in rats receiving either (a) perfused adrenal medulla; (b) isolated chromaffin cells; or (c) isolated chromaffin cells that were subsequently recombined with their nonchromaffin supporting cells. One year postimplantation, all graft types which included fibroblasts and endothelial cells were infiltrated with macrophages and demonstrated an abundance of cellular debris. No viable chromaffin cells were observed. In contrast, healthy tyrosine hydroxylase (TH) and dopamine beta hydroxylase (DβH) immunoreactive chromaffin cells survived for 1 yr posttransplantation when grafted in isolation from the nonchromaffin constituents of the adrenal medulla. Good xenograft survival was achieved in this group despite the fact that these rats were only immunosuppressed for 1 mo postimplantation. Grafted cells demonstrated morphological characteristics of chromaffin cells in situ and these implants were not accompanied by macrophage infiltration. These data demonstrate that long-term survival of chromaffin cells can be achieved following intra-striatal implantation and the viability of grafted chromaffin cells is dependent upon the removal of the nonchromaffin supporting cells.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Xenograft</ce:text></ce:keyword><ce:keyword><ce:text>Chromaffin cell</ce:text></ce:keyword><ce:keyword><ce:text>Intrastriatal transplantation</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Long-term viability of isolated bovine adrenal medullary chromaffin cells following intrastriatal transplantation</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Long-term viability of isolated bovine adrenal medullary chromaffin cells following intrastriatal transplantation</title></titleInfo><name type="personal"><namePart type="given">Sherry B.</namePart><namePart type="family">Schueler</namePart><affiliation>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jacqueline</namePart><namePart type="family">Sagen</namePart><affiliation>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">George D.</namePart><namePart type="family">Pappas</namePart><affiliation>Department of Anatomy and Cell Biology, University of Illinois School of Medicine, Chicago, IL 60612 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jeffrey H.</namePart><namePart type="family">Kordower</namePart><affiliation>To whom correspondence should be addressed.</affiliation><affiliation>Department of Neurological Sciences, Rush Presbyterian Medical Center, 2242 West Harrision Street, Chicago, IL 60612 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1994</dateIssued><dateValid encoding="w3cdtf">1994-09-06</dateValid><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Adrenal medullary grafts generally exhibit poor viability when grafted into the striatum. Previous work in our laboratory demonstrated that chromaffin cells can survive well for up to 2 mo following grafting into the intact rat striatum after cells are isolated from the nonchromaffin supporting cells (fibroblasts and endothelial cells) of the adrenal medulla. The aim of the present study was to assess the long-term viability of isolated bovine chromaffin cells following grafting into the intact rat striatum. The viability of grafted bovine adrenal medullary chromaffin cells was compared in rats receiving either (a) perfused adrenal medulla; (b) isolated chromaffin cells; or (c) isolated chromaffin cells that were subsequently recombined with their nonchromaffin supporting cells. One year postimplantation, all graft types which included fibroblasts and endothelial cells were infiltrated with macrophages and demonstrated an abundance of cellular debris. No viable chromaffin cells were observed. In contrast, healthy tyrosine hydroxylase (TH) and dopamine beta hydroxylase (DβH) immunoreactive chromaffin cells survived for 1 yr posttransplantation when grafted in isolation from the nonchromaffin constituents of the adrenal medulla. Good xenograft survival was achieved in this group despite the fact that these rats were only immunosuppressed for 1 mo postimplantation. Grafted cells demonstrated morphological characteristics of chromaffin cells in situ and these implants were not accompanied by macrophage infiltration. These data demonstrate that long-term survival of chromaffin cells can be achieved following intra-striatal implantation and the viability of grafted chromaffin cells is dependent upon the removal of the nonchromaffin supporting cells.</abstract><note type="content">Section title: Original contribution</note><subject><genre>Keywords</genre><topic>Xenograft</topic><topic>Chromaffin cell</topic><topic>Intrastriatal transplantation</topic></subject><relatedItem type="host"><titleInfo><title>Cell Transplantation</title></titleInfo><titleInfo type="abbreviated"><title>CTR</title></titleInfo><genre type="Journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199501</dateIssued></originInfo><identifier type="ISSN">0963-6897</identifier><identifier type="PII">S0963-6897(00)X0012-7</identifier><part><date>199501</date><detail type="issue"><title>Neural Transplantation into the CNS</title></detail><detail type="volume"><number>4</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>154</end></extent><extent unit="pages"><start>55</start><end>64</end></extent></part></relatedItem><identifier type="istex">49F400E7DD52410E715273CE694FB97B879231EE</identifier><identifier type="DOI">10.1016/0963-6897(94)00939-H</identifier><identifier type="PII">0963-6897(94)00939-H</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/49F400E7DD52410E715273CE694FB97B879231EE/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">MEDICINE, RESEARCH & EXPERIMENTAL</classCode><classCode scheme="WOS">TRANSPLANTATION</classCode><classCode scheme="WOS">CELL & TISSUE ENGINEERING</classCode><classCode scheme="WOS">CELL BIOLOGY</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000371 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000371 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= ParkinsonV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= ISTEX:49F400E7DD52410E715273CE694FB97B879231EE
|texte= Long-term viability of isolated bovine adrenal medullary chromaffin cells following intrastriatal transplantation
}}
| This area was generated with Dilib version V0.6.23. |  |